Composite

Part:BBa_K1426001

Designed by: Robert H. Newman   Group: iGEM14_Greensboro_Aggies   (2014-10-31)


pLac-RBS-RFP-term-XylR-pXyl-RBS-GFP-2term

This is a dual inducible expression system composed of an RFP color variant under the control of the Lac promotor (pLac) and a GFP color variant under the control of the Xyl promotor (pXyl). The addition of IPTG selectively promotes expression of RFP while the addition of xylose promotes the expression of GFP. The Xyl repressor (XylR), which binds pXyl and represses expression expression of genes under the control of that promoter, is also included in the part in order to repress GFP expression in the absence of xylose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1924
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3135


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Categories
Parameters
None